ZEB2 case 2

General

Cell Line

hPSCreg name IGGi005-A
Cite as:
IGGi005-A (RRID:CVCL_D6MR)
Alternative name(s)
ZEB2 case 2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
IGGi004-A
(ZEB2 case 1)
Donor diseases:
Mowat-Wilson syndrome
Last update 11th January 2024
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Provider

Generator IRCCS Istituto Giannina Gaslini (IGG)

External Databases

BioSamples SAMEA114786370
Cellosaurus CVCL_D6MR
Wikidata Q127382325

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Hirschsprung disease intellectual disability syndrome
  • Hirschsprung disease mental retardation syndrome
  • Hirschsprung disease-intellectual disability syndrome
  • Mowat-Wilson syndrome
  • microcephaly, intellectual disability, and distinct facial featrues, with or without Hirschprung disease
  • microcephaly, mental retardation, and distinct facial featrues, with or without Hirschprung disease
  • Hirschsprung disease-mental retardation syndrome
  • MOWS
  • intellectual disability, microcephaly, and distinct facial features with or without Hirschsprung disease
  • mental retardation, microcephaly, and distinct facial features with or without Hirschsprung disease
  • microcephaly, intellectual disability, and distinct Facial features, with or without Hirschsprung disease
  • microcephaly, mental retardation, and distinct Facial features, with or without Hirschsprung disease
show more synonyms

External Databases (Donor)

BioSamples SAMEA114786371

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. emilia.ricci@unimi.it
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? University of Milan
Approval number 11/23, 18-01-2023
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name PBMC
Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
SOX2
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Yellow: sox17; Mangenta: foxa2; Cyano: nuclei
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Yellow: brachyury; Mangenta: tbx6; Cyano: nuclei
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology
Yellow: nestin; Mangenta: tubb3; Cyano: nuclei

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 14
Karyotyping method: KaryoStat+ (Thermo Fisher Scientific, A52986)

Other Genotyping (Cell Line)