General

Cell Line
hPSCreg Name A standardised name that was automatically generated based on the cell line information. see Naming Tool	GENEAe013-A
Alternative name(s)	GENEA089
Cell line type	Human embryonic stem cell (hESC)
Last update	22nd May 2019
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Provider
Generator	Genea (GENEA) Contact: Biocells
Owner	Biocells
Distributors	Biocells
Derivation country	Australia
External Databases
BioSamples	SAMEA104012530
Cellosaurus	CVCL_M240
NIHhESC	NIHhESC-14-0247
CLO	CLO_0102701
Wikidata	Q54835661
General Information
Publications	McQuade LR et al. Proteomics of Huntington's disease-affected human embryonic stem cells reveals an evolving pathology involving mitochondrial dysfunction and metabolic disturbances. Journal of proteome research. 2014 Dec 5;13(12):5648-59. Dumevska B et al. Derivation of Huntington Disease affected Genea089 human embryonic stem cell line. Stem cell research. 2016 Mar;16(2):434-6.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: allowed Commercial use: allowed

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Huntington disease Huntington disease in the OLS The donor is a carrier of a disease-associated mutation. Synonyms Huntington chorea
Donor Relations
All cell lines of this donor's relatives	Has sister: GENEAe018-A Has sister: GENEAe019-A
External Databases (Donor)
BioSamples	SAMEA104012531

Ethics

Was the embryo established purely for research purposes?	No
Have both parents consented to the use of the embryo for ESC derivation?	Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Alternatives to consent are available?	Yes
Alternatives to consent	DOEE and ethics form sent separately as can't upload more than one file here.
Alternative consent approval number	NIHhESC-14-0247
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent the derivation of pluripotent stem cells?	No
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	NHMRC
Approval number	Licence 309710

hESC Derivation

Date of derivation	2012-05-30
Supernumerary embryos from IVF treatment?	Yes Separation of research and IVF treatment? Yes
PGD Embryo?	Yes

Culture Conditions

Medium

Other medium: Base medium: KO-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit FGF 20ng/mL %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
SSEA-4	Yes
TRA 1-60	Yes

PluriTest

Pluripotency Score	Novelty Score
39.28	1.2

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XX no abnormalities detected Passage number: 9
Other Genotyping (Cell Line)