GENEA090
GENEAe018-A
General
Cell Line |
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| hPSCreg name | GENEAe018-A |
| Cite as: | GENEAe018-A (RRID:CVCL_M241) |
| Alternative name(s) |
GENEA090
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
RCi004-A-1 (RCi004-A + HTT GC #H34-32_T34-23) Donor's gene variants: HTT Donor diseases: Huntington disease |
| Last update | 21st November 2019 |
| User feedback | |
Provider |
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| Generator |
Genea (GENEA)
Contact:
Biocells |
| Owner | Biocells |
| Distributors | |
| Derivation country | Australia |
External Databases |
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| BioSamples | SAMEA104130590 |
| Cellosaurus | CVCL_M241 |
| NIHhESC | NIHhESC-14-0245 |
| Wikidata | Q54835662 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Donor Relations |
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| All cell lines of this donor's relatives |
Has sister:
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External Databases (Donor) |
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| BioSamples | SAMEA104130591 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Additional files will be sent through separately as can't add more than 1 here. |
| Alternative consent approval number | NIHhESC-14-0245 |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | NHMRC |
| Approval number | Licence 309710 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | NHMRC |
| Approval number | Licence 309710 |
hESC Derivation
| Date of derivation | 2012-05-30 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
Yes |
Culture Conditions
| Medium |
Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| Pluripotency Score | Novelty Score | |
| 30.91 | 1.23 |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46 XX, no abnormalities detected
Passage number: 9
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Other Genotyping (Cell Line) |
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