GENEAe015-A

General#

Cell Line
hPSCreg Name	GENEAe015-A
Alternative name(s)	GENEA020
Cell line type	Human embryonic stem cell (hESC)
Last update	10th April 2017
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Provider
Generator	Genea (GENEA) Contact: Biocells
Owner	Biocells
Distributors	Biocells
External Databases
BioSamples	SAMEA104012534
Cellosaurus	CVCL_9029
General Information
Publications	Bradley CK et al. Derivation of Huntington's disease-affected human embryonic stem cell lines. Stem cells and development. 2011 Mar;20(3):495-502. McQuade LR et al. Proteomics of Huntington's disease-affected human embryonic stem cells reveals an evolving pathology involving mitochondrial dysfunction and metabolic disturbances. Journal of proteome research. 2014 Dec 5;13(12):5648-59. Dumevska B et al. Derivation of Huntington disease affected Genea020 human embryonic stem cell line. Stem cell research. 2016 Mar;16(2):430-3.
Is the cell line available in principle?	Available, without restrictions

Donor Information#

General Donor Information

Sex

female

Phenotype and Disease related information (Donor)

Diseases

A disease was diagnosed.

Huntington disease

Huntington disease in the OLS

PGD Undisclosure Case

The donor is a carrier of a disease-associated mutation.

Synonyms

Donor Relations

All cell lines of this donor's relatives

Has sister:

GENEAe020-A

External Databases (Donor)

BioSamples

SAMEA104012535

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation	2007-12-18
Supernumerary embryos from IVF treatment?	Yes Separation of research and IVF treatment? Yes
PGD Embryo?	Yes

Culture Conditions#

Medium

Other medium:

Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Supplements

Supplement	Amount	Unit
FGF		%

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
TRA 1-60	Yes
SSEA-4	Yes

PluriTest

Pluripotency Score	Novelty Score
27.51	1.43

Other pluripotency tests used

Teratoma positive, Alkaline Phosphatase stain positive.

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

Ectoderm

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XX no abnormalities detected
Other Genotyping (Cell Line)

GENEA020