GENEAe015-A

GENEA020

General#

Cell Line

hPSCreg Name GENEAe015-A
Alternative name(s)
GENEA020
Cell line type Human embryonic stem cell (hESC)
Last update 10th April 2017
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Owner Biocells
Distributors

External Databases

BioSamples SAMEA104012534
Cellosaurus CVCL_9029

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
PGD Undisclosure Case
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Huntington chorea

Donor Relations

All cell lines of this donor's relatives
Has sister:

External Databases (Donor)

BioSamples SAMEA104012535

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2007-12-18
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Pluripotency Score Novelty Score
27.51 1.43
Teratoma positive, Alkaline Phosphatase stain positive.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX no abnormalities detected

Other Genotyping (Cell Line)