GENEAe015-A

GENEA020

General

Cell Line

hPSCreg name GENEAe015-A
Cite as:
GENEAe015-A (RRID:CVCL_9029)
Alternative name(s)
GENEA020
Cell line type Human embryonic stem cell (hESC)
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Last update 21st November 2019
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Owner Biocells
Distributors

External Databases

BioSamples SAMEA104012534
Cellosaurus CVCL_9029
Wikidata Q54835582

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
PGD Undisclosure Case
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Huntington chorea

Donor Relations

All cell lines of this donor's relatives
Has sister:

External Databases (Donor)

BioSamples SAMEA104012535

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Genea Biocells
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? Yes
Alternatives to consent Genea020 was derived from embryonic outgrowth cells, originally developed for karyotyping, not from the embryo with fully informed consent. the PGD was performed on an undisclosure basis.only one document can be added here so other document will be emailed.
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? GENEA HREC
Approval number Meeting 13/9/2005
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? GENEA HREC
Approval number Meeting 13/9/2005

hESC Derivation

Date of derivation 2007-12-18
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions

Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Pluripotency Score Novelty Score
27.51 1.43
Teratoma positive, Alkaline Phosphatase stain positive.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX no abnormalities detected
Passage number: 55

Other Genotyping (Cell Line)