GENEAe020-A

GENEA019

General#

Cell Line

hPSCreg Name GENEAe020-A
Alternative name(s)
GENEA019
Cell line type Human embryonic stem cell (hESC)
Last update 10th April 2017

Provider

Generator Genea (GENEA)
Contact:
Biocells
Owner Biocells
Distributors Biocells
Derivation country Australia

External Databases

BioSamples SAMEA104013010
Cellosaurus CVCL_9028

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

All cell lines of this donor's relatives
Has sister:

External Databases (Donor)

BioSamples SAMEA104013011

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hESC Derivation#

Date of derivation 2007-12-18
Embryo stage 0
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique 0
Cell isolation 0
Cell seeding 0

Culture Conditions#

Medium Other medium:
Base medium: KO-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
FGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Pluripotency Score Novelty Score
22.97 1.42
Teratoma positive, Alkaline Phosphatase stain positive.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX no abnormalities detected

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)