Derivation of human embryonic stem cell line Genea019
Summary
The Genea019 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype, female Allele pattern and unaffected Htt CAG repeat length, compared to HD affected sibling Genea020. Pluripotency of Genea019 was demonstrated with 75% of cells expressing Nanog, 89% Oct4, 48% Tra1-60 and 85% SSEA4, a Pluritest Pluripotency score of 22.97, Novelty score of 1.42, tri-lineage teratoma formation and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination. Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.
| Authors | Dumevska B, Peura T, McKernan R, Goel D, Schmidt U |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2016 Mar;16(2):397-400 |
| PubMed | 27346002 |
| DOI | 10.1016/j.scr.2016.02.008 |