hPSCreg®
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RCi004-A-1

Registration Summary:

RCi004-A + HTT GC #H34-32_T34-23

RCi004-A-1

General

Cell Line
hPSCreg name RCi004-A-1
Cite as:
RCi004-A-1
Alternative name(s)
RCi004-A + HTT GC #H34-32_T34-23
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi004-A
(PDSC-10, RCi150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
RCi004-B
(RCi68, PDSC-3)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi001-A
(#1c8, CHDI-90002149)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi003-A
(#3c1, CHDI-90002151)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi005-A
(#5c4, CHDI-90002153)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi010-A
(#10c2, CHDI-90002158)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi020-A
(#20c2, CHDI-90002168)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi022-A
(#22c1, CHDI-90002170)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi026-A
(#26c3, CHDI-90002174)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi029-A
(#29c4, CHDI-90002177)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi031-A
(#31c1, CHDI-90002179)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi033-A
(#104c2, CHDI-90002181)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi034-A
(#105c1, CHDI-90002182)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi041-A
(#116c2, CHDI-90002189)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi048-A
(#125c2, CHDI-90002196)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi049-A
(#127c2, CHDI-90002197)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi055-A
(#133c5, CHDI-90002203)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi028-A
(#28c3, CHDI-90002176)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi035-A
(#108c5, CHDI-90002183)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi036-A
(#110c5, CHDI-90002184)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi038-A
(#112c7, CHDI-90002186)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi040-A
(#115c6, CHDI-90002188)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi045-A
(#121c6, CHDI-90002193)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi046-A
(#122c1, CHDI-90002194)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
ICGi018-A
(iHD38Q-3)
Donor's gene variants:
HTT
Donor diseases:
Huntington's Disease
ICGi018-B
(iHD38Q-2)
Donor's gene variants:
HTT
Donor diseases:
Huntington's Disease
ICGi018-C
(iHD38Q-1)
Donor's gene variants:
HTT
Donor diseases:
Huntington's Disease
ICGi007-A
(47Q-3Lf)
Donor's gene variants:
HTT
Donor diseases:
Huntington disease
IIMCBi006-B
(M-T2)
Donor diseases:
Huntington's Disease
GENEAe013-A
(GENEA089)
Donor diseases:
Huntington disease
GENEAe015-A
(GENEA020)
Donor diseases:
Huntington disease
GENEAe018-A
(GENEA090)
Donor diseases:
Huntington disease
GENEAe019-A
(GENEA091)
Donor diseases:
Huntington disease
IIMCBi006-A
(M-T1)
Donor diseases:
Huntington's Disease
INSRMe003-A
(STR-I-155-HD)
Donor diseases:
Huntington disease
RGIe091-A
(SI-186)
Donor diseases:
Huntington disease
CSSi004-A
(HD256.05 cl1)
Donor diseases:
Huntington disease
RCPCMi002-A
(HD76)
Donor diseases:
Huntington disease
RGIe092-A
(SI-187)
Donor diseases:
Huntington disease
RGIe098-A
(SI-194)
Donor diseases:
Huntington disease
KCLe009-A
(KCL012_HD3)
Donor diseases:
Huntington disease
Last update 20th December 2022
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Provider
Generator Bioneer (BION)
Distributors

External Databases
BioSamples SAMEA7462886

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Huntington disease
The donor is a carrier of a disease-associated mutation and not affected.
Stage
Pre-symptomatic at time of biopsy
Synonyms
  • Huntington chorea
Genetic variants
HTT (target)
4p16.3
NM_002111.7:c.52CAG[40]
Heterozygous
SCV000054533.1
DNA sample indicates one CAG repeat allele within the normal range (approximately 18 repeats) and one allele within the Huntington disease affected range (approximately 40 repeats) in the HTT gene.

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA3106020

Ethics

Also have a look at the ethics information for the parental line RCi004-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line RCi004-A.
Passage number reprogrammed 48

Reprogramming method
Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line RCi004-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Huntington Disease
Genetic modifications
Isogenic modification
HTT
Heterozygous
The original line is associated with Huntington's disease and has 20 CAGs on one allele and 40 on the other. The corrected line has 17 CAGs on one allele and 25 on the other allele. Note that there is also a difference in a proline repeat next to the CAG repeat: The original line has 14 prolines on the allele with the 20 CAGs and 11 prolines on the allele with the 40 CAGs. The corrected line has 14 prolines on the allele with the 17 CAGs and 11 prolines on the allele with the 25 CAGs