RCi004-A + HTT GC #H34-32_T34-23

General

Cell Line

hPSCreg name RCi004-A-1
Cite as:
RCi004-A-1
Alternative name(s)
RCi004-A + HTT GC #H34-32_T34-23
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 20th December 2022
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Provider

Generator Bioneer (BION)
Distributors

External Databases

BioSamples SAMEA7462886

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and not affected.
Stage
Pre-symptomatic at time of biopsy
Synonyms
  • Huntington chorea
Genetic variants
HTT (target)
4p16.3
NM_002111.7:c.52CAG[40]
Heterozygous
SCV000054533.1
DNA sample indicates one CAG repeat allele within the normal range (approximately 18 repeats) and one allele within the Huntington disease affected range (approximately 40 repeats) in the HTT gene.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3106020

Ethics

Also have a look at the ethics information for the parental line RCi004-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line RCi004-A.
Passage number reprogrammed 48

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line RCi004-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Genetic modifications
Isogenic modification
HTT
Heterozygous
The original line is associated with Huntington's disease and has 20 CAGs on one allele and 40 on the other. The corrected line has 17 CAGs on one allele and 25 on the other allele. Note that there is also a difference in a proline repeat next to the CAG repeat: The original line has 14 prolines on the allele with the 20 CAGs and 11 prolines on the allele with the 40 CAGs. The corrected line has 14 prolines on the allele with the 17 CAGs and 11 prolines on the allele with the 25 CAGs