|Cell line type
|Human embryonic stem cell (hESC)
(RCi004-A + HTT GC #H34-32_T34-23)
Donor's gene variants:
|4th September 2022
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Ontogeny of primordial germ cells
|* Is the cell line readily obtainable for third parties?
General Donor Information
Phenotype and Disease related information (Donor)
|A disease was diagnosed.
|Was the embryo established purely for research purposes?
|Have both parents consented to the use of the embryo for ESC derivation?
|Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?
|Was the consent voluntarily given?
|Alternatives to consent are available?
|Alternatives to consent
|Alternative consent approval number
|Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.
|Does consent explicitly allow the derivation of pluripotent stem cells?
|Does consent expressly prevent the derivation of pluripotent stem cells?
|How may genetic information associated with the cell line be accessed?
|Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?
|Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?
|Name of accrediting authority involved?
|Blastula with ICM and Trophoblast
|ZP removal technique
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Microbiology / Virus Screening
Karyotyping (Cell Line)
|Has the cell line karyotype been analysed?
Passage number: 31
Other Genotyping (Cell Line)