RCi68, PDSC-3

General#

Cell Line

hPSCreg Name RCi004-B
Alternative name(s)
RCi68, PDSC-3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Roslin Cells (RC)
Owner Censo Biotechnologies (CENSO)
Distributors

External Databases

BioSamples SAMEA3106205
Cellosaurus CVCL_9S48
ECACC 66540014
EBiSC RCi004-B

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
The donor is a carrier of a disease-associated mutation and not affected.
Stage
Pre-symptomatic at time of biopsy
Synonyms
  • Huntington chorea
Genetic variants
HTT (huntingtin) (target)
4p16.3
NM_002111.7:c.52CAG[40]
Heterozygous
SCV000054533.1
DNA sample indicates one CAG repeat allele within the normal range (approximately 18 repeats) and one allele within the Huntington disease affected range (approximately 40 repeats) in the HTT gene.
Disease associated phenotypes
  • Mutant gene: HTT

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3106020

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 45-49
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
GSC
No
GATA6
Yes
CXCR4
Yes
PITX1
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
GATA4
Yes
DCN
Yes
PECAM
No
PDGF
No
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
No
HES5
Yes
FOXG1
No
B-TUBULIN
No
NEUROD1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
14 out of 20 cells normal, 4 cells loss of chromosome 20 short arm and gain of long arm. 2 cells 46,XX,add(16)(p13.1)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes