47Q-3Lf

General#

Cell Line

hPSCreg Name ICGi007-A
Alternative name(s)
47Q-3Lf
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th June 2019
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Provider

Generator The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG)
Owner The Federal Research Center Institute of Cytology and Genetics The Siberian Branch of the Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA5275258
Cellosaurus CVCL_UJ80

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 25-29
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
The donor is a carrier of the Huntington's disease mutation inherited by the father. The mutation has been identified in both the father, the donor and the cell line.
The donor is a carrier of a disease-associated mutation and not affected.
Stage
The donor is not showing symptoms yet.
Synonyms
  • Huntington chorea
Genetic variants
HTT huntingtin (target)
4p16.3
Heterozygous
30658253
Mutation in the published line was detected by capillary electrophoresis. It was performed using capillary genetic analyzer ABI Prism 3130 (Applied Biosystems), size standard Liz 600; obtained data were analyzed using Data Collection software v3.0 and GeneMapper v. 4.0 (Applied Biosystems).
Disease associated phenotypes no phenotypes
Non-disease associated phenotypes
  • Cancer
Family history The donor's father had a heterozygous mutation in exon 1 of the HTT gene (45 CAG-repeats).

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5275617

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
Policy for access to genetic information Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Center of New Medical Technology in Akademgorodok
Approval number 21
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Center of New Medical Technology in Akademgorodok
Approval number 21
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell type
mononuclear cell
A cell having only one nucleus, especially: MONOCYTE.
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms
Age of donor (at collection) 25-29
Collected in 2016
Passage number reprogrammed 1

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones The selection of colonies was carried out according to morphological criteria. We selected flat monolayer colonies with tightly packed cells with high nuclear/cytoplasmic ratio. Embryonic stem cell-like clones were picked by a glass microcapillary.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout DMEM (Life Technologies)
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
NEAA 0.1 mM
GlutaMAX 2 mM
rhFGF basic 10 ng/ml
Penicillin 50 U/ml
Streptomycin 50 µg/ml
2-mercaptoethanol 0.1 mM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
Marker Present Absent
mCpG
OCT4
Morphology pictures
Morphology-ICGi007-A.tif
Morphology of ICGi007-A IPSC, the scale bar - 100 mcm
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
CK18
Yes
Morphology
ICGi007-A GATA6_g KRT18_r.tif
Immunofluorescent analysis for endoderm markers GATA6 (green signal) and Cytokeratin18 (red signal) in ICGi007-A IPSC, DAPI (blue signal), the scale bar - 100 mcm
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
collagen IV
Yes
vimentin
Yes
Morphology
ICGi007-A Vim_g Coll4_r.tif
Immunofluorescent analysis for ectoderm markers Vimentin (green signal) and Collagen IV (red signal) in ICGi007-A IPSC, DAPI (blue signal), the scale bar - 100 mcm
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Neurofilament 200
Yes
Morphology
ICGi007-A NF200_g.tif
Immunofluorescent analysis for marker ectoderm Neurofilament 200 (green signal), DAPI (blue signal), the scale bar - 100 mcm

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotyping and G-banding show ICGi007-A PSCs have a normal 46, XX karyotype.
Karyotyping method: G-Banding

Other Genotyping (Cell Line)