RCPCMi002-A

HD76

The cell line is not validated yet.

General

Cell Line

hPSCreg name RCPCMi002-A
Cite as:
RCPCMi002-A (RRID:CVCL_UK95)
Alternative name(s)
HD76
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi004-A-1
(RCi004-A + HTT GC #H34-32_T34-23)
Donor's gene variants:
HTT
Donor diseases:
Huntington disease
WAe009-A-1P
(IsoHD-30Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1Q
(IsoHD-65Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1R
(IsoHD-81Q-NGN2)
Donor diseases:
Huntington's disease
RCi004-B
(RCi68, PDSC-3)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
IIMCBi006-B
(M-T2)
Donor diseases:
Huntington's Disease
GENEAe013-A
(GENEA089)
Donor diseases:
Huntington disease
GENEAe015-A
(GENEA020)
Donor diseases:
Huntington disease
GENEAe018-A
(GENEA090)
Donor diseases:
Huntington disease
GENEAe019-A
(GENEA091)
Donor diseases:
Huntington disease
IIMCBi006-A
(M-T1)
Donor diseases:
Huntington's Disease
RGIe091-A
(SI-186)
Donor diseases:
Huntington disease
CHDIi001-A
(#1c8, CHDI-90002149)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi003-A
(#3c1, CHDI-90002151)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi005-A
(#5c4, CHDI-90002153)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi010-A
(#10c2, CHDI-90002158)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi020-A
(#20c2, CHDI-90002168)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi022-A
(#22c1, CHDI-90002170)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi026-A
(#26c3, CHDI-90002174)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi029-A
(#29c4, CHDI-90002177)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi031-A
(#31c1, CHDI-90002179)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi033-A
(#104c2, CHDI-90002181)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi034-A
(#105c1, CHDI-90002182)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi041-A
(#116c2, CHDI-90002189)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi048-A
(#125c2, CHDI-90002196)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi049-A
(#127c2, CHDI-90002197)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi055-A
(#133c5, CHDI-90002203)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
INSRMe003-A
(STR-I-155-HD)
Donor diseases:
Huntington disease
RCi004-A
(PDSC-10, RCi150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
WAe009-A-1H
(MYL3-KO)
Donor diseases:
Huntington's disease
WAe009-A-1I
(H9::GFP cyto-reporter line)
Donor diseases:
Huntington's disease
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
WAe009-A-1J
(H9::mcherry cyto-reporter line)
Donor diseases:
Huntington's disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
Donor diseases:
Huntington's disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
WAe009-A-36
(JPH2-KO)
Donor diseases:
Huntington's disease
WAe009-A-12
(H9_RB1ex3_C7)
Donor diseases:
Huntington's disease
Last update 22nd May 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Federal State Budgetary Institution Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency (RCPCM)
Derivation country Russia

External Databases

BioSamples SAMEA5230327
Cellosaurus CVCL_UK95
Wikidata Q98128874

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Huntington chorea

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

External Databases (Donor)

BioSamples SAMEA5230328

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Research Center of Neurology, Deputy Head of Institute S.N Illarioshkin +7 (495) 374-77-76
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Research Center of Neurology, Deputy Head of Institute S.N Illarioshkin +7 (495) 374-77-76
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Center of Neurology
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Name of accrediting authority involved?
Approval number
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General

Source cell line name skin fibroblast
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Methods used
Immunostaining, RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
TRA 1-81
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46
Passage number: 3
Karyotyping method: G-Banding

Other Genotyping (Cell Line)