KCL013_HD4

General

Cell Line

hPSCreg Name
KCLe010-A
Alternative name(s)
KCL013_HD4
Cell line type Human embryonic stem cell (hESC)
Last update 4th September 2022
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A247
NIHhESC NIHhESC-13-0214
CLO CLO_0102641
Wikidata Q54899661

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
The donor is affected.
Synonyms
  • Huntington chorea

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-13-0214
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? HFEA
Approval number

hESC Derivation

Date of derivation 2009-08-09
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 5
Stephenson 2007
ICM morphology E
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions

Surface coating Cellstart
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 8

Other Genotyping (Cell Line)