KCL013_HD4

General#

Cell Line

hPSCreg Name KCLe010-A
Alternative name(s)
KCL013_HD4
Cell line type Human embryonic stem cell (hESC)
Last update 27th October 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A247
NIHhESC NIHhESC-13-0214

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
The donor is affected.
Synonyms
  • Huntington chorea

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2009-08-09
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 5
Stephenson 2007
ICM morphology E
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions#

Surface coating Cellstart
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
FGF 2

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)