KCL012_HD3

General

Cell Line

hPSCreg name KCLe009-A
Cite as:
KCLe009-A (RRID:CVCL_A246)
Alternative name(s)
KCL012_HD3
Cell line type Human embryonic stem cell (hESC)
Similar lines
RCi004-A-1
(RCi004-A + HTT GC #H34-32_T34-23)
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HTT
Donor diseases:
Huntington disease
WAe009-A-1P
(IsoHD-30Q-NGN2)
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WAe009-A-1Q
(IsoHD-65Q-NGN2)
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WAe009-A-1R
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CHDIi002-A
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HTT, HTT
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Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
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HTT, HTT
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Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
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HTT, HTT
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Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi028-A
(#28c3, CHDI-90002176)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi035-A
(#108c5, CHDI-90002183)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi036-A
(#110c5, CHDI-90002184)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi038-A
(#112c7, CHDI-90002186)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi040-A
(#115c6, CHDI-90002188)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi045-A
(#121c6, CHDI-90002193)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi046-A
(#122c1, CHDI-90002194)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CSSi004-A
(HD256.05 cl1)
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Huntington disease
RGIe098-A
(SI-194)
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Huntington disease
CHDIi001-A
(#1c8, CHDI-90002149)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi003-A
(#3c1, CHDI-90002151)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi005-A
(#5c4, CHDI-90002153)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi010-A
(#10c2, CHDI-90002158)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi020-A
(#20c2, CHDI-90002168)
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HTT, HTT
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Huntington disease
CHDIi022-A
(#22c1, CHDI-90002170)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi026-A
(#26c3, CHDI-90002174)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi029-A
(#29c4, CHDI-90002177)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi031-A
(#31c1, CHDI-90002179)
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HTT, HTT
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Huntington disease
CHDIi033-A
(#104c2, CHDI-90002181)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi034-A
(#105c1, CHDI-90002182)
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HTT, HTT
Donor diseases:
Huntington disease
CHDIi041-A
(#116c2, CHDI-90002189)
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HTT, HTT
Donor diseases:
Huntington disease
ICGi033-B
(77Q-9)
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Huntington disease
CHDIi048-A
(#125c2, CHDI-90002196)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
ICGi033-C
(77Q-20)
Donor diseases:
Huntington disease
CHDIi049-A
(#127c2, CHDI-90002197)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi055-A
(#133c5, CHDI-90002203)
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HTT, HTT
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Huntington disease
VUBe005-A
(VUB05_HD)
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Huntington disease
INSRMe003-A
(STR-I-155-HD)
Donor diseases:
Huntington disease
RCi004-A
(PDSC-10, RCi150)
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HTT, HTT
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Huntington disease
RCi004-B
(RCi68, PDSC-3)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
IIMCBi006-B
(M-T2)
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Huntington's Disease
KCLe010-A
(KCL013_HD4)
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Huntington disease
GENEAe013-A
(GENEA089)
Donor diseases:
Huntington disease
GENEAe015-A
(GENEA020)
Donor diseases:
Huntington disease
GENEAe018-A
(GENEA090)
Donor diseases:
Huntington disease
GENEAe019-A
(GENEA091)
Donor diseases:
Huntington disease
IIMCBi006-A
(M-T1)
Donor diseases:
Huntington's Disease
Last update 4th September 2022
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A246
NIHhESC NIHhESC-13-0213
Wikidata Q54899660

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Huntington chorea

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-13-0213
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? NRES
Approval number

hESC Derivation

Date of derivation 2009-08-09
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 5
Stephenson 2007
ICM morphology E
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions

Surface coating Cellstart
Feeder cells Human Foreskin Fibroblast
Cellfinder Ont Id: CELDA_000001419
O2 Concentration 5 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
FGF 2

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 5

Other Genotyping (Cell Line)