Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene


The KCL012 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (46 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays. Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.

Authors Jacquet L, Hewitson H, Wood V, Kadeva N, Cornwell G, Codognotto S, Hobbs C, Stephenson E, Ilic D
Journal Stem cell research
Publication Date 2016 Mar;16(2):264-7
PubMed 27345979
PubMed Central PMC4823764
DOI 10.1016/j.scr.2016.01.012

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