Generation of GPAM knockout human embryonic stem cell line SYSUe-008-A using CRISPR/Cas9


GPAM (glycerol-3-phosphateacyltransferase1) is a mitochondrial enzyme that catalyze an essential step in glycerolphospholipids and triacylglycerol biosynthesis process. Loss-of-function mutation of GPAM has been shown to lead to hypomyelination of corticospinal tract in cerebral palsy patient. To model this rare disease with human brain organoid, we generated a GPAM knockout human embryonic stem cell line SYSUe-008-A by CRISPR/cas9. The GPAM knockout cell line maintains a normal karyotype and shows comparable level of pluripotent stem cell marker expression and differentiation potential as wild-type human embryonic stem cells. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Sun C, Li B, Yang M, Guo R, Yuan S, Wang J, Hu H
Journal Stem cell research
Publication Date 2021 May;53:102303
PubMed 33831647
DOI 10.1016/j.scr.2021.102303

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