Generation of GPAM knockout human embryonic stem cell line SYSUe-008-A using CRISPR/Cas9
Summary
GPAM (glycerol-3-phosphateacyltransferase1) is a mitochondrial enzyme that catalyze an essential step in glycerolphospholipids and triacylglycerol biosynthesis process. Loss-of-function mutation of GPAM has been shown to lead to hypomyelination of corticospinal tract in cerebral palsy patient. To model this rare disease with human brain organoid, we generated a GPAM knockout human embryonic stem cell line SYSUe-008-A by CRISPR/cas9. The GPAM knockout cell line maintains a normal karyotype and shows comparable level of pluripotent stem cell marker expression and differentiation potential as wild-type human embryonic stem cells. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Sun C, Li B, Yang M, Guo R, Yuan S, Wang J, Hu H |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102303 |
| PubMed | 33831647 |
| DOI | 10.1016/j.scr.2021.102303 |