Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression


The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector-mediated tetracycline-inducible β-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the β-catenin protein, encoded by the CTNNB1 gene, after exposure to doxycycline (Dox). CTNNB1 gene expression was confirmed by quantitative PCR (qPCR) and immunofluorescence assays. Further characterization confirmed that the NERCe003-A-1 cell line expresses typical pluripotency markers and has the ability to form the three germ layers both in vitro and in vivo. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang Y, Yu J, Liu L, Li W, Duan X, Peng Y, Zeng S, Ouyang Q, Lu G, Lin G, Sun Y
Journal Stem cell research
Publication Date 2018 Apr;28:61-65
PubMed 29428849
DOI 10.1016/j.scr.2018.01.020

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