Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression
Summary
The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector-mediated tetracycline-inducible β-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the β-catenin protein, encoded by the CTNNB1 gene, after exposure to doxycycline (Dox). CTNNB1 gene expression was confirmed by quantitative PCR (qPCR) and immunofluorescence assays. Further characterization confirmed that the NERCe003-A-1 cell line expresses typical pluripotency markers and has the ability to form the three germ layers both in vitro and in vivo. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Wang Y, Yu J, Liu L, Li W, Duan X, Peng Y, Zeng S, Ouyang Q, Lu G, Lin G, Sun Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2018 Apr;28:61-65 |
| PubMed | 29428849 |
| DOI | 10.1016/j.scr.2018.01.020 |