Generation of a NONO homozygous knockout human induced pluripotent stem cell line by CRISPR/Cas9 editing


The non-POU domain containing octamer-binding gene (NONO) encodes a member of a small family of RNA-binding and DNA-binding proteins, whose variants can cause intellectual disability and congenital heart defects. In this study, we generated a homozygous NONO knockout (NONO-KO) induced pluripotent stem cell (iPSC) line (CMUi002-A-1) using the CRISPR/Cas9-based genome editing system. The gene-edited line had a normal karyotype, expressed pluripotency markers, and was able to differentiate into all three germ layers in vivo. This cell line will provide a platform to study the pathogenic mechanisms of noncompaction cardiomyopathy and neurocyte dysfunction related to NONO mutations. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Yi T, Bai R, Sun H, Lan F, Zhang H, He Y
Journal Stem cell research
Publication Date 2020 Jul 8;47:101893
PubMed 32717574
DOI 10.1016/j.scr.2020.101893

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