XDCMHi001-A

General#

Cell Line

hPSCreg Name XDCMHi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 20th November 2019
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Provider

Generator Xiaoshan District Chinese Medicine Hospital (XDCMH)
Owner Xiaoshan District Chinese Medicine Hospital (XDCMH)
Distributors
Derivation country China

External Databases

BioSamples SAMEA5070575
Cellosaurus CVCL_WT97

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Medical science research
Research: allowed
Clinical: allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
ankylosing spondylitis
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Marie-Struempell Disease
  • MARIE STRUEMPELL DIS
  • Rheumatoid Spondylitis
  • Ankylosing Spondyloarthritides
  • Spondylarthritis, Ankylosing
  • BECHTEREWS DIS
  • Marie Struempell Disease
  • Ankylosing Spondylarthritis
  • BECHTEREW DIS
  • Spondyloarthritides, Ankylosing
  • Ankylosing Spondylarthritides
  • Spondylitis, Rheumatoid
  • Bechterew's Disease
  • Bechterews Disease
  • Bechterew Disease
  • Ankylosing Spondyloarthritis
  • Spondyloarthritis, Ankylosing
  • Spondylitis, Ankylosing
  • Spondylarthritides, Ankylosing
  • Spondylarthritis Ankylopoietica
  • ankylosing spondylitis
  • Marie-Strumpell disease
  • Bekhterev's disease
  • Bekhterev syndrome
show more synonyms
Disease associated phenotypes
  • pathological osteogenesis
  • Morning stiffness
  • Back pain
Family history His father suffering from ankylosing spondylitis.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5712207

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: 527356976@qq.com
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink 527356976@qq.com
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? No
a non-profit company? No
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Urine
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms
Source cell origin
Urine
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
Immunostaining, RT-PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells Mouse embryonic fibroblast
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM /F12 Medium
Main protein source: Knock-out serum replacement
Serum concentration: 0 %
Supplements
bFGF 5 ng/ml
Non-Essential Amino Acids Solution 1 %
GlutaMAX 1 %
2-Mercaptoethanol 100 µM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)