SA202

General#

Cell Line

hPSCreg Name CEBe018-A
Alternative name(s)
SA202
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Cellartis AB Sweden (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B302

General Information

Publications
Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2002-11-01
Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating MEF
Medium Other medium:
Base medium: DMEM/F12 with Glutamax
Main protein source: Anti-Human-Serum(Sigma)
Supplements
Fetal Calf Serum %
hrbFGF %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
NANOG
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)