Generation of two induced pluripotent stem cell lines SPPHi001-A and SPPHi002-A from a Huntington's disease family of Southwestern China


The Huntington's disease (HD) is still a devastating neurological disease without a specific cure. In this report, the peripheral blood mononuclear cells (PBMCs) from two members of a HD family from Southwestern China were reprogrammed into hiPSC lines by an episomal and non-integrating Sendai virus delivery method. The induced HD-hiPSC lines, SPPHi001-A and SPPHi002-A, were expanded and validated for their stem cell-like pluripotency, normal karyotyping, and capability of in vitro differentiation into three germ-layers. These newly generated HD hiPSC lines from Chinese HD patients enable us to further elucidate the mechanism of HD and discover its specific treatment. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Li B, Ye F, Chen L, Yang X, Zhang J, Luo Y, Xu J, Luo Y, Wang S
Journal Stem cell research
Publication Date 2021 Jan 4;50:102149
PubMed 33429129
DOI 10.1016/j.scr.2020.102149

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