SI-158

General#

Cell Line

hPSCreg Name RGIe066-A
Alternative name(s)
SI-158
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Reproductive Genetics Institute (RGI)
Derivation country United States

External Databases

Cellosaurus CVCL_B582

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
beta thalassemia
The donor is affected.

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation#

Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
TRA 2-39
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)