OZ-8
IMHe011-A
General
Cell Line |
|
| hPSCreg name | IMHe011-A |
| Cite as: | IMHe011-A (RRID:CVCL_B030) |
| Alternative name(s) |
OZ-8
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
|
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator | ISTANBUL MEMORIAL HOSPITAL (IMH) |
| Derivation country | Turkey |
External Databases |
|
| Cellosaurus | CVCL_B030 |
| Wikidata | Q54897575 |
General Information |
|
| Publications |
|
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Disease associated phenotypes |
|
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Local Institutional Review Board |
hESC Derivation
| Date of derivation | 2005-01-30 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
Yes |
| Expansion status |
4
|
| ICM morphology |
A
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Enzymatic |
| Cell isolation | Mechanical |
| Cell seeding | Embryo |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
|||||||||
| Passage method | Mechanically | |||||||||
| O2 Concentration | 21 % | |||||||||
| CO2 Concentration | 6 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum Serum concentration: 15 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 18
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.