UPSFRi004-A-1

PB05-1

General

Cell Line

hPSCreg name UPSFRi004-A-1
Cite as:
UPSFRi004-A-1 (RRID:CVCL_C348)
Alternative name(s)
PB05-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UPSFRi004-A
(PB05)
Donor diseases:
beta thalassemia
IMHe011-A
(OZ-8)
Donor diseases:
beta thalassemia
RGIe072-A
(SI-164)
Donor diseases:
beta thalassemia
UPSFRi002-A
(PB04)
Donor diseases:
beta thalassemia
RGIe066-A
(SI-158)
Donor diseases:
beta thalassemia
Last update 14th November 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Université Paris-Sud 11 (UPSFR)
Contact:
ESTeam Paris Sud
Derivation country France

External Databases

Cellosaurus CVCL_C348
Wikidata Q54991766

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.

Ethics

Also have a look at the ethics information for the parental line UPSFRi004-A .
Alternatives to consent
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General

The source cell information can be found in the parental cell line UPSFRi004-A.

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF
2-mercaptoethanol

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
HESCA-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)

Genetic Modification