HPSI0313i-cesj_1

General#

Cell Line

hPSCreg Name WTSIi108-A
Alternative name(s)
HPSI0313i-cesj_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA2398465
HipSci HPSI0313i-cesj_1
Cellosaurus CVCL_AE71
ECACC 77650127

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398550
HipSci HPSI-cesj

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
27.781 1.58 https://www.ebi.ac.uk/ega/datasets/EGAD00010000775

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0313i-cesj_1
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 1
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Text file with probe intensities
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Expression array
https://ega-archive.org/datasets/EGAD00010001143
GenomeStudio text file
Expression array
https://ega-archive.org/datasets/EGAD00010001143
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment