Establishment of induced pluripotent stem cell line CSUASOi003- A from an autosomal recessive retinitis pigmentosa patient carrying compound heterozygous mutations in CRB1 gene


Crumbs homologue 1 (CRB1) mutations have been found in retinitis pigmentosa (RP) patients lead to severe retinal dystrophies. The human induced pluripotent stem (iPS) cell line CSUASOi003-A derived from peripheral blood mononuclear cells (PBMCs) of a patient carrying two heterozygous mutations (2249G>A p.G750D and c.2809G>A p.A937T) in CRB1 gene was generated by non-integrative reprogramming technology. Pluripotency and differentiation capacity were assessed by immunocytochemistry and quantitative polymerase chain reaction (qPCR). The RP patient-specific iPS cell line provide a powerful model for evaluating the pathological phenotypes of the disease. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Zhou Y, Ding C, Xia S, Jing Y, Mao S, Liu J, Chen J, Chan HF, Tang S, Chen J
Journal Stem cell research
Publication Date 2020 Apr;44:101742
PubMed 32244200
DOI 10.1016/j.scr.2020.101742

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