Generation of a bank of clinical-grade, HLA-homozygous iPSC lines with high coverage of the Spanish population
Summary
Here, we present the first clinical-grade, iPSC haplobank in Spain made from CD34 + cells from seven cord blood units homozygous for the most common HLA-A, HLA-B and HLA-DRB1 haplotypes within the Spanish population. We describe their generation by transduction with Sendai viral vectors and their GMP-compliant expansion and banking. These haplolines will constitute starting materials for advanced therapy medicinal product development (ATMP). © 2023. The Author(s).
| Authors | Kuebler B, Alvarez-Palomo B, Aran B, Castaño J, Rodriguez L, Raya A, Querol Giner S, Veiga A |
|---|---|
| Journal | Stem cell research & therapy |
| Publication Date | 2023 Dec 13;14(1):366 |
| PubMed | 38093328 |
| PubMed Central | PMC10720139 |
| DOI | 10.1186/s13287-023-03576-1 |