Generation of a human induced pluripotent stem cell line with Cas9 driven by Tet-on operator via AAVS1 safe harbor gene-editing
Human induced pluripotent stem (iPS) cells expressing Cas9 protein are valuable for the pathogenic mechanism study and drug discovery. These cells can be efficiently induced to differentiate into disease cell models with specific mutations through adding designed sgRNAs. Here, we generated a human gene-editable iPS cell line by gene editing method that Cas9 gene driven by Tet-on operator was perfectly integrated into the human AAVS1 safe harbor locus. The established Cas9 expression iPS cell line named as WMUi013-A can express endogenous pluripotent markers, has the ability to differentiate into the three germ layers, and possesses a normal karyotype. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Ding Y, Wang L, Ji W, Chen Z, Wang D, Chen C, Tong H, Han Z, Niu C, Chu M, Huang J, Guo X|
|Journal||Stem cell research|
|Publication Date||2020 Dec;49:102064|