LSCTRi006-A

LSCTR-LiPSC-4, i4

General

Cell Line

hPSCreg Name
LSCTRi006-A
Alternative name(s)
LSCTR-LiPSC-4, i4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th November 2022
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Provider

Generator Laboratory of Stem Cells and Tissue Regeneration (LSCTR)

External Databases

Cellosaurus CVCL_UM09
Wikidata Q95983156
BioSamples SAMEA111456267
CLO CLO_0104133

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA111491274

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: National Biobank of Korea, Korea Disease Control and Prevention Agency, Republic of Korea
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Korea University Institutional Review Board
Approval number 1040548-KU-IRB-16-1-A-1(E-A-1)
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
lymphoblastoid cell line
Human cell line from tissue infected with Epstein-Barr virus, resembling a lymphoblast.

Reprogramming method

Vector type Non-integrating
Vector Episomal plasmid
Is reprogramming vector detectable?
Yes
Methods used
PCR
Notes on reprogramming vector detection pCXLE-hOCT3/4-shp53 (hOCT3/4, shp53), pCXLE-hUL (L-myc, Lin28), pCXLE-hSK (Sox2, Klf4)

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
T brachyury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Nestin
Yes

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX, normal

Other Genotyping (Cell Line)