Generation of three induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying TNNI3 mutations


Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease with a prevalence of about 0.2%. HCM is typically caused by mutations in genes encoding sarcomere or sarcomere-associated proteins. Here, we characterized induced pluripotent stem cell (iPSC) lines generated from the peripheral blood mononuclear cells of three HCM patients each carrying c.433C > T, c.610C > T, or c.235C > T mutation in the TNNI3 gene by non-integrated Sendai virus. All of the three lines exhibited normal morphology, expression of pluripotent markers, stable karyotype, and the potential of trilineage differentiation. The cardiomyocytes differentiated from these iPSC lines can serve as useful tools to model HCM in vitro. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhao SR, Shen M, Lee C, Zha Y, Guevara JV, Wheeler MT, Wu JC
Journal Stem cell research
Publication Date 2021 Nov 12;57:102597
PubMed 34798544
DOI 10.1016/j.scr.2021.102597

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