hiPS45

General#

Cell Line

hPSCreg Name EPFLi005-A
Alternative name(s)
hiPS45
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd August 2018
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Provider

Generator Swiss Federal Institute of Technology (EPFL)
Distributors

External Databases

BioSamples SAMEA104012167
EBiSC EPFLi005-A
ECACC 66540577
Cellosaurus CVCL_VD38

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104012328

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type (free text) CD34+ Hematopoietic stem cell

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
LIN28A
Yes
DPPA2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)