Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9


Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13+/-) and a CDH13 null mutant (CDH13-/-) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Vitale MR, Zöller JEM, Jansch C, Janz A, Edenhofer F, Klopocki E, van den Hove D, Vanmierlo T, Rivero O, Nadif Kasri N, Ziegler GC, Lesch KP
Journal Stem cell research
Publication Date 2021 Mar;51:102169
PubMed 33486346
DOI 10.1016/j.scr.2021.102169

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