An integration-free induced pluripotent stem cell line from a 42-year-old female donor with the APOE-ε2/ε2 allele
Summary
The APOE 4 allele remains the primary genetic risk factor for sporadic Alzheimer's disease, whereas the APOE 2 allele emerges as a protective factor. Therapeutic approaches in murine models with human APOE alleles, such as modulating APOE levels and converting isoforms, show efficacy. However, there is a lack of in vitro APOE2-mutant human neuronal models. Hence, in this study, we generated human induced pluripotent stem cells (hiPSCs) from the peripheral blood mononuclear blood cells (PMBC) of a 42-year-old female donor carrying the APOE-ε2/ε2 allele. The newly generated hiPSC displayed normal karyotype and could differentiate into three germ layers. Besides, they retained their original genotype and expressed pluripotency markers. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Wang L, Guo R, Zhang X, Zhou Z, Yao Akogo H, Fu Z, Song Y, Li M, Ma J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Oct;80:103506 |
| PubMed | 39094506 |
| DOI | 10.1016/j.scr.2024.103506 |