HAD-C 106
HADe009-A
General#
Cell Line |
|
| hPSCreg Name | HADe009-A |
| Alternative name(s) |
HAD-C 106
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 10th April 2017 |
| User feedback | |
Provider |
|
| Generator | Hadassah University Hospital (HAD) |
| Owner | Hadassah University Hospital (HAD) |
| Distributors | |
| Derivation country | Israel |
External Databases |
|
| Cellosaurus | CVCL_B862 |
| NIHhESC | NIHhESC-11-0125 |
General Information |
|
| Publications | |
| Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | No diseases known |
| Is the medical history available upon request? | Medical history known |
| Is clinical information available? | No health conditions known |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 2009-01-16 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
4
|
| ICM morphology |
A
|
| Trophectoderm morphology |
b
|
| Cell isolation | Laser |
| Cell seeding | Isolated ICM |
| Derived under xeno-free conditions? |
Yes |
| Derivation under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions#
| Surface coating | Gelatin |
| Feeder cells |
umbilical cord fibroblasts |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DMEM
Main protein source: Human Serum Serum concentration: 20 % |
Characterisation#
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
XY Normal
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
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