Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology
Summary
Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent inherited renal disease, characterized by multiple cysts that can lead to kidney failure resulting in end-stage renal disease. ADPKD is mainly caused by mutations in either the PKD1 and PKD2 genes, encoding for polycystin-1 and polycystin-2, respectively. In order to clarify the disease mechanisms, here we describe the generation of two isogenic induced pluripotent stem cell (iPSC) lines in which the PKD2 gene was deleted using CRISPR/Cas9 technology. The PKD2-/- iPSCs expressed the main pluripotency markers, were able to differentiate into the three germ layers and had a normal karyotype. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Trionfini P, Ciampi O, Romano E, Benigni A, Tomasoni S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Jan;42:101667 |
| PubMed | 31830647 |
| DOI | 10.1016/j.scr.2019.101667 |