General

Cell Line
hPSCreg name	HVRDe009-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	HVRDe009-A-1 (RRID:CVCL_A4DR)
Alternative name(s)	HuES9DGCR8+/-
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	20th May 2026
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Provider
Generator	RCNS-Institute of Molecular Life Sciences (RCNS)
External Databases
Cellosaurus	CVCL_A4DR
Wikidata	Q105509552
General Information
Publications	Reé D et al. Establishing a human embryonic stem cell clone with a heterozygous mutation in the DGCR8 gene. Stem cell research. 2021 Jan;50:102134. Reé D et al. Partial Disturbance of Microprocessor Function in Human Stem Cells Carrying a Heterozygous Mutation in the DGCR8 Gene. Genes. 2022 Oct 23;13(11).
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Additional restrictions: An MTA must also be concluded with the original creator of the cell line
Subclone of	HVRDe009-A

Genetic Modification

Disease/phenotype related modifications

DGCR8 wt Allele Synonyms C22orf12 Chromosome 22 Open Reading Frame 12 Gene DGCR8 Microprocessor Complex Subunit wt Allele DGCR8 wt Allele DGCRK6 DiGeorge Syndrome Critical Region Gene 8 Gy1 Pasha Gene show more synonyms show less synonyms Genetic modifications Type of modification Gene knock-out Chromosome location 22 Delivery method CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	Yes
External Databases (Donor)
BioSamples	SAMEA8944198

Ethics

Also have a look at the ethics information for the parental line HVRDe009-A .

Is there an MTA available for the cell line?	Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	Yes
Constraints for use or distribution	For research use only and an MTA must also be concluded with the original creator of the cell line.

hESC Derivation

The source cell information can be found in the parental cell line HVRDe009-A.

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzymatically Accutase
O2 Concentration	21 %
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Unknown

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line HVRDe009-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Karyotyping method: G-Banding
Other Genotyping (Cell Line)