chHES-8-KO-P53
NERCe003-A-3
General
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA104132951 |
Ethics
Also have a look at the ethics information for the parental line
NERCe003-A
.
| Alternatives to consent |
hESC Derivation
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The source cell information can be found in the parental cell line
NERCe003-A.
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Culture Conditions
| Feeder cells |
human embryonic fibroblast |
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| Passage method | Mechanically | ||||||||||||
| O2 Concentration | 5 % | ||||||||||||
| CO2 Concentration | 5 % | ||||||||||||
| Medium |
Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46，XY
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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