NERCe003-A-3

chHES-8-KO-P53

General#

Cell Line

hPSCreg Name NERCe003-A-3
Alternative name(s)
chHES-8-KO-P53
Cell line type Human embryonic stem cell (hESC)
Last update 31st January 2018
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Provider

Generator National Engineering and Research Center of Human Stem Cell (NERC)
Owner National Engineering and Research Center of Human Stem Cell (NERC)

External Databases

BioSamples SAMEA994658
Cellosaurus CVCL_VF84

General Information

Is the cell line available in principle?
No
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA104132951

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hESC Derivation#

Date of derivation 2017-11-10
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Feeder cells human embryonic fibroblast
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine %
NEAA
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)
Yes
Genetic modifications not related to a disease
Gene knock-out
17p13.1
CRISPR-associated (CRISPR/Cas) System