chHES-8-KO-P53
NERCe003-A-3
General#
Cell Line |
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hPSCreg Name | NERCe003-A-3 |
Alternative name(s) |
chHES-8-KO-P53
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 22nd May 2019 |
User feedback | |
Provider |
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Generator | National Engineering and Research Center of Human Stem Cell (NERC) |
Owner | National Engineering and Research Center of Human Stem Cell (NERC) |
External Databases |
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BioSamples | SAMEA994658 |
Cellosaurus | CVCL_VF84 |
CLO | CLO_0102906 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
No |
Subclone of |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA104132951 |
Ethics#
Also have a look at the ethics information for the parental line
NERCe003-A
.
Alternatives to consent |
hESC Derivation#
Date of derivation | 2017-11-10 |
Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
PGD Embryo? |
No |
Culture Conditions#
Feeder cells |
human embryonic fibroblast |
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Passage method | Mechanically | ||||||||||||
O2 Concentration | 5 % | ||||||||||||
CO2 Concentration | 5 % | ||||||||||||
Medium |
Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XY
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Other Genotyping (Cell Line) |
Genetic Modification#
Genetic modifications not related to a disease |
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