General#

Cell Line
hPSCreg Name A standardised name that was automatically generated based on the cell line information. see Naming Tool	NERCe003-A-3
Alternative name(s)	chHES-8-KO-P53
Cell line type	Human embryonic stem cell (hESC)
Last update	22nd May 2019
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Provider
Generator	National Engineering and Research Center of Human Stem Cell (NERC)
Owner	National Engineering and Research Center of Human Stem Cell (NERC)
External Databases
BioSamples	SAMEA994658
Cellosaurus	CVCL_VF84
CLO	CLO_0102906
Wikidata	Q54930414
General Information
Publications	Li Y et al. Generation of NERCe003-A-3, a p53 compound heterozygous mutation human embryonic stem cell line, by CRISPR/Cas9 editing. Stem cell research. 2019 Jan;34:101371.
* Is the cell line readily obtainable for third parties?	No
Subclone of	NERCe003-A

Donor Information#

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA104132951

Ethics#

Also have a look at the ethics information for the parental line NERCe003-A .

Alternatives to consent

hESC Derivation#

The source cell information can be found in the parental cell line NERCe003-A.

Culture Conditions#

Feeder cells	human embryonic fibroblast
Passage method	Mechanically
O2 Concentration	5 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: D/F 12 Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements Supplement Amount Unit L-Glutamine % NEAA bFGF

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
TRA 1-60	Yes
TRA 1-81	Yes
POU5F1 (OCT-4)	Yes
SOX2	Yes

hPSC Scorecard™

Self-renewal

Positive

Endoderm

Positive

Mesoderm

Positive

Ectoderm score

Positive

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46，XY
Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease

Type of modification Gene knock-out Chromosome location 17p13.1 Delivery method CRISPR-associated (CRISPR/Cas) System