chHES-8-KO-P53
NERCe003-A-3
General#
Cell Line |
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| hPSCreg Name | NERCe003-A-3 |
| Alternative name(s) |
chHES-8-KO-P53
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 31st January 2018 |
| User feedback | |
Provider |
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| Generator | National Engineering and Research Center of Human Stem Cell (NERC) |
| Owner | National Engineering and Research Center of Human Stem Cell (NERC) |
External Databases |
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| BioSamples | SAMEA994658 |
| Cellosaurus | CVCL_VF84 |
General Information |
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| Is the cell line available in principle? |
No |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA104132951 |
Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 2017-11-10 |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
Culture Conditions#
| Feeder cells |
human embryonic fibroblast |
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| Passage method | Mechanically | ||||||||||||
| O2 Concentration | 5 % | ||||||||||||
| CO2 Concentration | 5 % | ||||||||||||
| Medium |
Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46，XY
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Other Genotyping (Cell Line) |
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Genetic Modification#
| Genetic modifications not related to a disease |
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