NERCe003-A-2

chHES-8-pINDUCER10-tet-shβ-catenin

General

Cell Line

hPSCreg Name
NERCe003-A-2
Alternative name(s)
chHES-8-pINDUCER10-tet-shβ-catenin
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator National Engineering and Research Center of Human Stem Cell (NERC)

External Databases

BioSamples SAMEA104132953
CLO CLO_0102896

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104132951

Ethics

Also have a look at the ethics information for the parental line NERCe003-A .
Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the ethical committee of Reproductive and Genetic Hospital of CITIC-Xiangya
Approval number 2001-01

hESC Derivation

The source cell information can be found in the parental cell line NERCe003-A.

Culture Conditions

Feeder cells human embryonic fibroblast
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
L-Glutamine %
NEAA
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Alkaline Phosphatase
Yes
SOX2
Yes
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
CTNNB1-knockdown (target)
Transgene expression
Viral
Lentivirus