chHES-8-pINDUCER10-tet-shβ-catenin
NERCe003-A-2
General
Cell Line |
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| hPSCreg name | NERCe003-A-2 |
| Cite as: | NERCe003-A-2 |
| Alternative name(s) |
chHES-8-pINDUCER10-tet-shβ-catenin
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
NERCe003-A-1 (chHES-8-pINDUCER20-tet-CTNNB1) NERCe003-A (chHES-8) UCSFi001-A-25 (AICS-0058-067) HVRDe009-A-2 (HUES9 CTNNB1 3X FLAG) NERCe003-A-3 (chHES-8-KO-P53) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | National Engineering and Research Center of Human Stem Cell (NERC) |
External Databases |
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| BioSamples | SAMEA104132953 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA104132951 |
Ethics
Also have a look at the ethics information for the parental line
NERCe003-A
.
| Was the embryo established purely for research purposes? | Yes |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | the ethical committee of Reproductive and Genetic Hospital of CITIC-Xiangya |
| Approval number | 2001-01 |
hESC Derivation
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The source cell information can be found in the parental cell line
NERCe003-A.
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Culture Conditions
| Feeder cells |
human embryonic fibroblast |
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| Passage method | Mechanically | ||||||||||||
| O2 Concentration | 5 % | ||||||||||||
| CO2 Concentration | 5 % | ||||||||||||
| Medium |
Other medium:
Base medium: D/F 12
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| Alkaline Phosphatase |
Yes |
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| SOX2 |
Yes |
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Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46，XY
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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