HVRDe009-A-2

HUES9 CTNNB1 3X FLAG

General#

Cell Line

hPSCreg Name
HVRDe009-A-2
Alternative name(s)
HUES9 CTNNB1 3X FLAG
Cell line type Human embryonic stem cell (hESC)
Last update 26th May 2021
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Provider

Generator Arizona State University (ASU)
Derivation country United States

External Databases

BioSamples SAMEA9005187
CLO CLO_0103343

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA8944198

Ethics#

Also have a look at the ethics information for the parental line HVRDe009-A .

hESC Derivation#

Date of derivation 2019-02-03
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
Cell isolation Immunosurgery
Cell seeding Single cell and ICM isolated

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line HVRDe009-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX, inv(9)
Passage number: 65
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
CTNNB1 (target)
Transgene expression
3p22.1
HUES9 line with 3X FLAG at C-terminal CTNNB1 gene. 3X FLAG sequence inserted using CRISPR/Cas9. Plasmids introduced using electroporation.
CRISPR-associated (CRISPR/Cas) System