General

iPSC Line
hPSCreg name	HVRDe009-A-2
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	HVRDe009-A-2 (RRID:CVCL_A8QB)
Alternative name(s)	HUES9 CTNNB1 3X FLAG
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	HVRDe009-A (HuES9) UCSFi001-A-25 (AICS-0058-067) NERCe003-A-1 (chHES-8-pINDUCER20-tet-CTNNB1) NERCe003-A-2 (chHES-8-pINDUCER10-tet-shβ-catenin)
Last update	4th November 2021
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Provider
Generator	Arizona State University (ASU)
Derivation country	United States
External iPSC Databases
BioSamples	SAMEA9005187
Cellosaurus	CVCL_A8QB
Wikidata	Q108820516
General iPSC Information
Publications	Cutts J et al. Generation of 3X FLAG-tagged human embryonic stem cell (hESC) line to study WNT-induced β-catenin DNA interactions (HVRDe009-A-2). Stem cell research. 2021 Dec;57:102586.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	HVRDe009-A

iPSC Genetic Modification

Genetic modifications not related to a disease

CTNNB1 (target) Type of iPSC Modification Transgene expression Chromosome location 3p22.1 Free text HUES9 line with 3X FLAG at C-terminal CTNNB1 gene. 3X FLAG sequence inserted using CRISPR/Cas9. Plasmids introduced using electroporation. Delivery method CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	Yes
External Databases (Donor)
BioSamples	SAMEA8944198

Ethics

Also have a look at the ethics information for the parental line HVRDe009-A .

hESC Derivation

The source cell information can be found in the parental cell line HVRDe009-A.

iPSC Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzymatically Accutase
CO2 Concentration	5 %
Medium	Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?	Yes

iPSC Characterisation

No characterisation data could be found for this subclone. Please open parental cell line HVRDe009-A .

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46XX, inv(9) REPORT_CLG-36498.pdf Passage number: 65 Karyotyping method: G-Banding
Other Genotyping (iPSC Line)