hESBGN-04 (BG04)
VIACe004-A
General
Cell Line |
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| hPSCreg name | VIACe004-A |
| Cite as: | VIACe004-A (RRID:CVCL_9731) |
| Alternative name(s) |
hESBGN-04 (BG04)
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
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| Generator |
Novocell, Inc. (VIAC)
Contact:
Stem Cell Engineering unit |
| Derivation country | United States |
External Databases |
|
| Cellosaurus | CVCL_9731 |
| Wikidata | Q54796228 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
5
|
| ICM morphology |
C
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
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