CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation


The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and UKAi002-A-1, respectively) were generated. CXCL4KO iPS cells showed deletion of exon 1 and complete loss of CXCL4 protein. Pluripotency of iPS cells was confirmed by expression of pluripotency markers and trilineage differentiation. CXCL4KO iPS cells are expected to provide a valuable tool for investigating the role of CXCL4 in human diseases. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Boehnke J, Atakhanov S, Toledo MAS, Schüler HM, Sontag S, Chatain N, Koschmieder S, Brümmendorf TH, Kramann R, Zenke M
Journal Stem cell research
Publication Date 2021 Aug;55:102490
PubMed 34391098
DOI 10.1016/j.scr.2021.102490

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