UKAi002-B-1

PV1 JAK2 V617F het : CXCL4 KO

General#

Cell Line

hPSCreg Name
UKAi002-B-1
Alternative name(s)
PV1 JAK2 V617F het : CXCL4 KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd April 2021
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Provider

Generator Universitätsklinikum Aachen (UKA)
Owner Universitätsklinikum Aachen (UKA)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA8686795
CLO CLO_0103288
Cellosaurus CVCL_A7GX
Wikidata Q107117215

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Polycythemia Vera
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Polycythemia Rubra Vera
  • polycythemia vera
  • Polycythemia rubra vera
  • Polycythemia vera
  • Polycythemia Vera
show more synonyms
Genetic variants
JAK2 (target)
9p24.1
NM_001322194.1:c.1849G>T
NP_001309123.1:p.Val617Phe
Mosaic
VCV000014662.13
The donor peripheral blood cells (primary sample) used for reprogramming and iPSC generation are heterogeneous and genetically mosaic. Therefore the two iPSC lines from this donor (UKAi002-A and UKAi002-B) have different allele frequencies than the primary sample for the mutations tested.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA8394204

Ethics#

Also have a look at the ethics information for the parental line UKAi002-B .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Integrated DNA Technologies (IDT)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

The source cell information can be found in the parental cell line UKAi002-B.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Selection criteria for clones ES cell like morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryo fibroblasts
Passage method Enzymatically
Collagenase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Polycythemia Vera
Synonyms
  • Polycythemia Rubra Vera
  • polycythemia vera
  • Polycythemia rubra vera
  • Polycythemia vera
  • Polycythemia Vera
show more synonyms
Genetic modifications
CXCL4, PV4 (target)
Gene knock-out
4q13.3
In the CXCL4 (PF4) gene deletions of 402 bp (allele 1) and 399 bp (allele 2) were generated by CRISPR/Cas9 editing (ITD DNA ALT-R) targeting CXCL4 (PF4) exon 1, which lead to the absence of CXCL4 (PF4) protein.
CRISPR-associated (CRISPR/Cas) System