PV1 JAK2 V617F het : CXCL4 KO
UKAi002-B-1
General
Cell Line |
|
| hPSCreg name | UKAi002-B-1 |
| Cite as: | UKAi002-B-1 (RRID:CVCL_A7GX) |
| Alternative name(s) |
PV1 JAK2 V617F het : CXCL4 KO
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UKAi002-B (PBMNC2_hiPS_009, PV1 JAK2 V617F het) Donor's gene variants: JAK2, JAK2 Donor diseases: Polycythemia Vera UKAi002-A (PBMNC2_hiPS_007, PV1 JAK2) Donor's gene variants: JAK2, JAK2 Donor diseases: Polycythemia Vera UKAi003-A (hsc4_hiPS_021, PV2 JAK2 V617F hom) Donor's gene variants: JAK2, JAK2 Donor diseases: Polycythemia Vera |
| Last update | 22nd April 2021 |
| User feedback | |
Provider |
|
| Generator |
Universitätsklinikum Aachen (UKA)
Contact:
Universitätsklinikum Aachen (UKA) |
| Owner | Universitätsklinikum Aachen (UKA) |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA8686795 |
| Cellosaurus | CVCL_A7GX |
| Wikidata | Q107117215 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA8394204 |
Ethics
Also have a look at the ethics information for the parental line
UKAi002-B
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Integrated DNA Technologies (IDT) |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
UKAi002-B.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | ES cell like morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin | ||||||
| Feeder cells |
mouse embryo fibroblasts |
||||||
| Passage method |
Enzymatically
Collagenase
|
||||||
| O2 Concentration | 21 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: Knock out DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
||||||
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
||||||
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
||||||
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46 XX
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.