SHCDNi002-A

SHCDN002

General

Cell Line

hPSCreg name SHCDNi002-A
Cite as:
SHCDNi002-A (RRID:CVCL_YZ99)
Alternative name(s)
SHCDN002
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 11th May 2020
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Provider

Generator Shanghai Children's Hospital (SHCDN)
Owner Department of Neurology (BCHNEU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA6878082
Cellosaurus CVCL_YZ99
Wikidata Q98132502

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

none

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 1-4

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Keller syndrome
  • Opitz-Kaveggia syndrome
Genetic variants
x
NM_005120 c.1249-1G>C
NP_005111.2 p?
Heterozygous
de novo
Please explain briefly the supporting evidence
Please explain briefly the supporting evidence
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • X-linked intellectual disability with marfanoid habitus
Disease associated phenotypes
  • delayed developmental milestones; a tall forehead;low ear position;prominent nasal bridge;short philtrum
Family history None
Is the medical history available upon request? A one-year-old female patient was brought to our department with the chief complaints of mental retardation. She was delivered at term with a birth weight of 3000 grams. There was no history of suffocation at birth nor problems during pregnancy. The paren
Is clinical information available? The occipito-frontal circumference was 47 centimeters. The area of the anterior fontanel was 2 cm * 2 cm. The patient had distinctive facial characteristics: a tall forehead, low ear position, prominent nasal bridge, and short philtrum.She could raise her

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
The result displays a normal karyotype(46, XX),without detectable abnormalities.
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA6878085

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai Children’s Hospital.
Approval number REK 2017R021-F01
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai Children’s Hospital.
Approval number REK 2017R021-F01
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? thermo fisher

hIPSC Derivation

General

Source cell type
A preparation of autologous peripheral blood mononuclear cells (PBMCs).
Synonyms
  • Autologous Peripheral Blood Mononuclear Cell
  • Autologous PBMC
  • Autologous PBMCs
  • Autologous Peripheral Blood Mononuclear Cells
  • Therapeutic PBMCs
show more synonyms
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms
Age of donor (at collection) 1-4
Collected in 2019
Source cell line vendor Shanghai Children's Hospital
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
Protein

Other

Selection criteria for clones cells are small and present a compact group
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method ACCUTASE
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
AFP
Yes
PAX6
Yes
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
The result displays a normal karyotype(46, XX),without detectable abnormalities.
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes