SHCDNi003-A

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name SHCDNi003-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th July 2020
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Provider

Generator Shanghai Children's Hospital (SHCDN)
Owner Shanghai Children's Hospital (SHCDN)
Distributors
Derivation country China

External Databases

BioSamples SAMEA7044788
Cellosaurus CVCL_ZL96
CLO CLO_0102548

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 10-14

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Allan-Herndon-Dudley Syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • MCT8-Specific Thyroid Hormone Cell Membrane Transporter Deficiency
  • Monocarboxylate Transporter 8 Deficiency
  • Allan-Herndon-Dudley Syndrome
Genetic variants
X
NM_006517.4:c.1026+1G>A
Heterozygous
de novo
Family history None
Is the medical history available upon request? A one-year-old Chinese Han male infant with AHDS who presented with developmental delay, hypertonia, and often tilted his head back. Furthermore, he showed elevated serum FT3, decreased serum FT4 and T4.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7044789

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai Children’s Hospital
Approval number REK 2017R021-F01
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai Children’s Hospital
Approval number REK 2017R021-F01
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Autologous Peripheral Blood Mononuclear Cells
A preparation of autologous peripheral blood mononuclear cells (PBMCs).
Synonyms
  • Autologous Peripheral Blood Mononuclear Cell
  • Autologous PBMC
  • Autologous PBMCs
  • Autologous Peripheral Blood Mononuclear Cells
  • Therapeutic PBMCs
show more synonyms
Age of donor (at collection) 10-14
Collected in 2019
Source cell line vendor Shanghai Children's Hospital
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
Protein

Other

Selection criteria for clones cells are small and present a compact group
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
AFP
Yes
PAX6
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
The result displays a normal karyotype(46, XY),without detectable abnormalities.
Karyotyping method: G-Banding

Other Genotyping (Cell Line)