Generation of three induced pluripotent stem cell lines (RAUi001-A, RAUi001-B and RAUi001-C) from peripheral blood mononuclear cells of a healthy Armenian individual
Summary
The study of pathological processes in cells carrying mutations should be carried out in comparison with a healthy control group. Familial Mediterranean fever (FMF), which is caused by a mutation in the MEFV gene, is predominantly found in people of Armenian nationality with the prevalence of 14-100 per 10000. We have obtained induced pluripotent stem cells (iPSCs) from Armenian healthy patient, which will be included as a control group in the study of this disease. iPSCs rapidly proliferate in colonies of cells with a typical pluripotent-like morphology, have a normal karyotype (46,XX). iPSCs express pluripotency markers (OCT4, SOX2, TRA-1-60, NANOG) and are able to give derivatives of three germ layers. Copyright © 2023. Published by Elsevier B.V.
| Authors | Grigor'eva EV, Malakhova AA, Ghukasyan L, Hayrapetyan V, Atshemyan S, Vardanyan V, Zakian SM, Zakharyan R, Arakelyan A |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Sep;71:103147 |
| PubMed | 37354743 |
| DOI | 10.1016/j.scr.2023.103147 |