CUBi001-A
General#
Cell Line |
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hPSCreg Name | CUBi001-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator |
Charité - Universitätsmedizin Berlin (CUB)
Contact:
Berlin Institute of Health (BIH) |
Distributors | |
Derivation country | Germany |
External Databases |
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BioSamples | SAMEA103992830 |
Cellosaurus | CVCL_IT59 |
General Information |
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Publications | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
46,XY
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External Databases (Donor) |
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BioSamples | SAMEA103992831 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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Age of donor (at collection) | neonate |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-1 |
No |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
Pluripotency Score | Novelty Score | Link to microarray data |
32.75 | 1.3 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5145 |
Transcriptome Characterisation
Differentiation Potency
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XY
Karyotyping method:
KaryoLite BoBs
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Other Genotyping (Cell Line) |
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