Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder


We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Bozaoglu K, Gao Y, Stanley E, Fanjul-Fernández M, Brown NJ, Pope K, Green CC, Vlahos K, Sourris K, Bahlo M, Delatycki M, Scheffer I, Lockhart PJ
Journal Stem cell research
Publication Date 2019 Aug;39:101516
PubMed 31415975
DOI 10.1016/j.scr.2019.101516

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