Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8)
Summary
The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Siehler J, Blöchinger AK, Akgün M, Wang X, Shahryari A, Geerlof A, Lickert H, Burtscher I |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Dec 16;50:102126 |
| PubMed | 33373890 |
| DOI | 10.1016/j.scr.2020.102126 |