Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human
Dermal fibroblasts obtained from a 43-year-old healthy man were successfully transformed into induced pluripotent stem cells (iPSCs) by employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and l-MYC. The iPSCs showed a normal karyotype and exhibited the potential to differentiate into three germ layers in a teratoma assay, which is often used to assess the pluripotency of stem cells. This iPSC line may be subsequently used for drug screens, biological tissue engineering, and cell transplantations. Copyright © 2020. Published by Elsevier B.V.
|Authors||Gao Y, Wang Y, Wang Z, Sun H, Zhang R, Yang J, Liu Y, Liu H, Zhang Q, Zhang S, Shi C, Xu Y, Song B|
|Journal||Stem cell research|
|Publication Date||2020 Jan 25;43:101717|