Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human
Summary
Dermal fibroblasts obtained from a 43-year-old healthy man were successfully transformed into induced pluripotent stem cells (iPSCs) by employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and l-MYC. The iPSCs showed a normal karyotype and exhibited the potential to differentiate into three germ layers in a teratoma assay, which is often used to assess the pluripotency of stem cells. This iPSC line may be subsequently used for drug screens, biological tissue engineering, and cell transplantations. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Gao Y, Wang Y, Wang Z, Sun H, Zhang R, Yang J, Liu Y, Liu H, Zhang Q, Zhang S, Shi C, Xu Y, Song B |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Mar;43:101717 |
| PubMed | 32007761 |
| DOI | 10.1016/j.scr.2020.101717 |