Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human


Dermal fibroblasts obtained from a 43-year-old healthy man were successfully transformed into induced pluripotent stem cells (iPSCs) by employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and l-MYC. The iPSCs showed a normal karyotype and exhibited the potential to differentiate into three germ layers in a teratoma assay, which is often used to assess the pluripotency of stem cells. This iPSC line may be subsequently used for drug screens, biological tissue engineering, and cell transplantations. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Gao Y, Wang Y, Wang Z, Sun H, Zhang R, Yang J, Liu Y, Liu H, Zhang Q, Zhang S, Shi C, Xu Y, Song B
Journal Stem cell research
Publication Date 2020 Mar;43:101717
PubMed 32007761
DOI 10.1016/j.scr.2020.101717

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