Generation of two induced pluripotent stem cell (iPSC) lines (BBANTWi006-A, BBANTWi007-A) from Brugada syndrome patients carrying an SCN5A mutation
Summary
Brugada syndrome (BrS) is an inherited primary electrical disorder of the heart. 25% of BrS patients carry a mutation in the SCN5A gene, encoding the cardiac specific voltage-gated sodium channel Nav1.5. Here we report two iPSC lines (BBANTWi006-A, BBANTWi007-A) of a brother and a sister carrying an SCN5A mutation (c.4813 + 3_4813 + 6dupGGGT) causing BrS. iPSCs were generated from dermal fibroblasts and reprogrammed with the Cytotune®-iPS 2.0 Sendai Reprogramming Kit (Invitrogen). The generated iPSCs showed a normal karyotype, expressed pluripotency markers, were differentiated into cells of the three germ layers and carried the original genotype. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Simons E, Nijak A, Loeys B, Alaerts M |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102719 |
| PubMed | 35247843 |
| PubMed Central | PMC8924004 |
| DOI | 10.1016/j.scr.2022.102719 |