Generation of a PPM1A-deficient human induced pluripotent stem cell line using CRISPR-Cas9 technology
Summary
PPM1A is a member of the serine/threonine protein phosphatase family. It can bind to a variety of proteins to dephosphorylate them, and extensively regulates many life activities such as cell growth, cell stress, immune response, and tumor formation. Here we constructed a human induced pluripotent stem cell (hiPSC) line with knockout of PPM1A using CRISPR/Cas9-mediated gene targeting. This cell line exhibits normal karyotype, pluripotency, and trilineage differentiation potential, which could provide a useful cellular resource for exploring the mechanism of PPM1A in regulating downstream signaling pathways and explore the application of PPM1A in anti-tumor and anti-infection. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Guo X, Zhao K, Zhang Y, Zhou T, Pan G |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103420 |
| PubMed | 38643711 |
| DOI | 10.1016/j.scr.2024.103420 |